北京方程生物
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人前心钠肽（PRO-ANP）酶联免疫分析
试剂盒使用说明书
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Human Pro Atrial Natriuretic Peptide
FOR RESEARCH USE ONLY
Drug Names
Generic Name：Human Pro Atrial Natriuretic Peptide (PRO-ANP) ELISA
Kit.
Purpose
This kit allows for the determination of PRO-ANP concentrations in
Human serum, cell culture supernatant, and other biological fluids.
Principle of the assay
The kit assay Human PRO-ANP level in the sample，use Purified Human
PRO-ANP to coat microtiter plate wells, make solid-phase antibody, then add
PRO-ANP to wells, Combined PRO-ANP antibody which With HRP labeled ,
become antibody - antigen - enzyme-antibody complex, after washing
Completely, Add TMB substrate solution,TMB substrate becomes blue color At
HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric
acid solution and the color change is measured spectrophotometrically at a
wavelength of 450 nm. The concentration of PRO-ANP in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
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Materialsprovided with the kit
Materials provided
with the kit
48determinations 96 determinations Storage
User manual 1 1
Closure plate
membrane
2 2
Sealed bags 1 1
Microelisa stripplate 1 1 2-8℃
Standard：180ng/L 0.5ml×1 bottle 0.5ml×1 bottle 2-8℃
1. The kit takes out from the refrigeration environment should be balanced
15-30 minutes in the room temperature, ELISA plates coated if has not use
up after opened, the plate should be stored in Sealed bag.
2. washing buffer will Crystallization separation, it can be heated the water
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helps dissolve when dilute . Washing does not affect the result.
3. add Sample with sampler Each step, And proofread its accuracy frequently,
avoids the experimental error. add sample within 5 mins, if the number of
sample is much , recommend to use Volley .
4. if the testing material content is excessively higher (The sample OD is
bigger than the first standard well ),please dilute Sample (n-fold), Please
diluente and multiplied by the dilution factor.（×n×5）.
5. Closure plate membrane only limits the disposable use, to avoid
cross-contamination.
6. The substrate evade the light preservation.
7. Please according to use instruction strictly, The test result determination
must take the microtiter plate reader as a standard.
8. All samples, washing buffer and each kind of reject should according to
infective material process.
9. Do not mix reagents with those from other lots.
Calculate
Assay range
Take the standard density as the horizontal,
the OD value for the vertical ,draw the standard
curve on graph paper, Find out the corresponding
density according to the sample OD value by the
Sample curve, multiplied by the dilution multiple,
or calculate the straight line regression equation
of the standard curve with the standard density
and the OD value ,with the sample OD value in
the equation, calculate the sample density,
multiplied by the dilution factor, the result is the
This chartis for reference only
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5ng/L -150ng/L
Storage and validity
1．Storage： 2-8℃.
2．validity： six months.